The Maynooth subdivision begins in Trenton at mile 0, the junction with the Deseronto and Picton subdivisions. The Maynooth subdivision was constructed as the Central Ontario railway through to Eldorado by 1884 and eventually on to Wallace by 1911.
The Hastings Heritage Trail now occupies the right-of-way north of Anson (mile post 15.39) and the junction with the Campbellford subdivision. Abandonment from Belmar through Maynooth took place in 1985. The line between Trenton and Belmar was abandoned in 1985.
The Trans-Canada Trail shares the path through to Bonarlaw (mile post 24.98). In 2004, the ballast was still deep and biking or running was difficult.